expression of citrus tristeza virus coat protein gene in escherichia coli

Expression of Citrus tristeza virus coat protein gene in Escherichia coli

Citrus tristeza virus (CTV) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. Considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in Iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in Iran, as one of the major citr...

Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli

cloning and expression of recombinant coat protein gene of turnip curly top virus in escherichia coli

turnip curly top virus (tctv) is a member of the newly established genus turncurtovirus, family geminiviridae. development of an efficient detection system is necessary to study the biological characteristics of viruses. due to the superior efficacy of the serological methods for detection of plant viruses, in this research, with the aim to facilitate the process of immunological detection of t...

Cloning and expression of coat protein gene of the most prevailing citrus tristeza virus isolate in Northern Iran

Citrus tristeza is one of the most destructive diseases of citrus and its causing agent CTV was introduced from Japan to northern Iran in the late 1960s. After about three decades of restriction to originally infected Satsuma trees, natural spread of CTV has being undertaken and melon aphid (Aphis gossypii Glov.) was confirmed as a potent vector in these areas. Regarding the specificity of aphi...

Expression of Prunus Necrotic Ringspot Virus Coat Protein in E. coli

Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...

serological methods to confirm expression of coat protein gene from an iranian isolate of cucumber mosaic virus in escherichia coli

conclusions this is the first report of expression of cmv cp gene in iran, which is important for the preparation of anti-cmv antibody and paving the way for the use of the virus coat protein as a nanomaterial. results the identity of the expressed protein was confirmed by immunoassays such as western blot, dot-immunobinding assay (diba) and enzyme-linked immunosorbent assay (elisa) by the use ...